Zeiss Elyra 7 lattice SIM
The Zeiss Elyra 7 with Lattice structured illumination microscopy (SIM) is a camera-based super-resolution system able to generate three-dimensional images at incredibly high frame rates. The system consists of an inverted microscope suitable for live cell imaging as well as fixed samples.
The new Lattice SIM technology of Elyra 7 combines very gentle imaging and superfast optical sectioning of 3D samples. Instead of grid lines, the system applies a novel spot illumination pattern which optimises the light efficiency and for fast and sensitive super-resolution imaging of live and fixed specimen. The new technique can be applied to uncover new mechanistic details, to quantify finest subcellular structures in large field of views and to study fast dynamic cellular processes.
- 3D SIM super-resolution imaging
- Gentle and very fast live cell imaging
- Time series capture
- Emission fingerprinting
- Co–localisation studies
- 2D PALM
- 2D STORM
Advantage:
- 3D super-resolution imaging with 120 nm lateral resolution
- Gentle imaging for less bleaching due to more efficient illumination pattern
- Super-fast imaging with no loss of image quality at frame rates up to 255 fps at 512 x 512 pixels
- Deeper sample penetration due to reduced contribution from out of focus light
- Large field of view - 1280 x 1280 pixels, 82 micrometre field diameter (63x oil)
- Simultaneous aquisition of two channels
- Flexibility to detect samples with non-standard emission wavelengths
- Axio observer 7 inverted stand
- Incubator XL dark and top stage incubation (full environmental chamber, temperature and CO2 control)
- Motorized Pieze XY scanning stage
- Z-Piezo stage insert
- 2 camera ports or one camera port with Duolink
- Objectives:
- EC Plan-Neofluar 10x/0.30 WD=5.2 M27
- LD LCI PApo 25x/0,8 Imm Corr
- Plan-APO 40x/1.4 Oil (UV) VIS-IR
- alpha Plan-Apochromat 63x/1.46
- C-Apochromat 63x/1.20 W Corr UV-VIS-IR
- Laser Lines:
| Laser | Excitation Lines (nm) | Colour of fluorophores | Examples of fluorophores |
|---|---|---|---|
| HR diode | 405 (50 mW) | Blue | DAPI / Alexa 350 |
| HR diode | 488 (100 mW) | Green / Cyan and Yellow | GFP / Alexa 488 / FITC |
| HR DPSS | 561 (100 mW) | Red | DsRed / TagRFR / Alexa 568 |
| HR diode | 642 (100 mW) | Far-Red |
Alexa 633 / CY5 |
ZEISS Elyra 7 Illumination and Detection:
- Fibre coupled solid state or diode pumped solid state lasers
- Lasers shared between Lattice SIM and single molecule localisaction microscopy (SMLM)
- PCO edge sCMOS camera (Lattice SIM, SMLM, SIM Apotome mode)
Software:
• ZEN (black edition)
• Lattice SIM / SIM Apotome module
• PALM / dSTORM module
