Technologies
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Flow cytometers
At EXCC, we possess three Aurora spectral analysers that are capable of high dimensional flow cytometry with two (Aurora A/B) situated in the Streatham campus and another at the Environmental and Sustainability Institute (ESI) in Penryn (Aurora P). We also have an Attune NxT and Cytoflex LX for high throughput analysis.
Aurora A
- 5 lasers, 64 fluorescence and 3 Scatter channels
- Enhanced Small Particle (ESP) detection through scatter
- Measure 40 colours simultaneously
- Autofluorescence correction
Applications: Identification of cells, Functional analysis of cells, microbiology, marine and freshwater research, environmental analysis, small particle analysis.
Aurora B/P ASL
- 5 lasers, 64 fluorescence and 3 scatter channels
- Automated Sample Loader (ASL) for high throughput
- Measure 40 colours simultaneously
- Autofluorescence correction
Situated in Streatham (Aurora B) and Penryn (Aurora P).
Applications: Identification of cells, Functional analysis of cells, microbiology, marine and freshwater research, environmental analysis, small particle analysis, high throughput screening
Attune NxT
- 5 lasers, 14 fluorescence and 2 scatter channels
- Plate loader for high throughput
- Acoustic focusing positions cells in central axis to enable higher sensitivity
- High flow rate to rapidly analyse samples
- Measure up to 14 colours simultaneously
Applications: Identification of cells, Functional analysis of cells, microbiology, marine and freshwater research, environmental analysis, small particle analysis, high throughput screening.
Cytoflex LX
- 7 lasers, 21 fluorescence and 2 scatter channels
- Plate loader for high throughput
- Measure up to 21 colours simultaneously
Imaging cytometers
We have 2 flow cytometry imaging platforms that combine the speed of a flow cytometer with functionality of a microscope, as well as a benchtop fluorescence microscope.
ImageStreamX MKII
- High resolution imaging of 1000 cells per second
- 5 lasers, allowing 10 fluorescent channels, side-scatter and brightfield images per cell
- Advanced software for quantification of fluorescence and morphological parameters
Applications: Identification of cells, Functional analysis of cells, microbiology, marine and freshwater research, environmental analysis, small particle analysis
FlowCam 8400
- Combine speed of a flow cytometer with functionality of a microscope
- Brightfield color images with 488 nm laser excitation
- Image objects ranging from 2 μm to 1 mm
Applications: Microbiology, Marine and freshwater research, Environmental Water Quality Assessment
Evos M5000 microscope
- Digital inverted benchtop fluorescence microscope
- User-defined 4-color fluorescence, transmitted light, and color images
Applications: Identification of cells, functional analysis of cells
Cell sorters
EXCC operates several cutting-edge flow cytometry sorters for the purification of particles (e.g. cells, chromosomes, viruses) for downstream applications such as metabolic analysis, in vitro assays, omic measurements or single cell genomic approaches such as a single cell RNA sequencing.
Bigfoot Spectral
- 6 lasers, 56 Fluorescence, 5 Scatter
- Integrated biocontainment
- Small Particle detector
- Multi-way sorting (up to 6-way, virtually 18-way)
Sort into 96-, 384- and 1536 well plates, petri dishes, microscope slides, and 10x chips
FACSAria Fusion
- 4Laser, 16 Fluorescence, 2 scatter
- BSLII Biosafety Cabinet
- Multi-way sorting (up to 4-way)
Sort into 96-well plates, petri dish, microscope slides, and 10x chips
» More about the BD FACSAria Fusion
FACSymphony S6 SE Spectral
- 5 lasers, 48 Fluorescence, 2 scatter
- BSLII Biosafety Cabinet
- Multi-way sorting (up to 6-way)
Sort into 96- and 384- well plates, petri dishes, microscope slides, and 10x chips
Omics
We have multiple platforms to enable omics-based measurements of RNA, protein and other biological molecules.
10X ChromiumX
Microfluidic system to partition cells into individual droplets, where RNA molecules are prepared for sequencing
Prepare hundreds to tens of thousands of cells per run
Applications: Omics technology, Identification of cells, Functional analysis of cells
Luminex FLEXMAP 3D
Performs nucleic acid assays, immunoassays, receptor-ligand assays, and enzymatic assays
96- and 384-well formats
Applications: Screening platform
Tissue Cytometry
MACSima Imaging Cytomics System
- High-resolution fluorescence imaging
- High-content characterisation with up to 400 markers for same tissue slice
Applications: Identification of cells, Location of cells in tissues, Functional analysis of cells
