DNA sample submission guidelines
Whole Genome Sequencing
- Sample Type: Genomic DNA (RNA-free)
- Sample Purity
- OD260/280: 1.8 - 2.0
- OD260/230: 2.0 - 2.2
- Quality checked on: gel or Genomic Tapestation. DNA should not be degraded.
- Recommended Quantity checked by fluorimetry (like the Qubit): Samples normalised between 0.1 ng/μl to 40 ng/μl. The higher the concentration, the lower the number of PCR cycles performed.
- Volume: 60 μl
- Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
- Shipment Method: Ice pack or dry ice, overnight
Metagenomics (DNA PCR free) Sequencing
- Sample Type: Genomic DNA (RNA-free)
- Sample Purity
- OD260/280: 1.8 - 2.0
- OD260/230: 2.0 – 2.2
- Quality checked on: gel or Genomic Tapestation.
- Recommended Quantity checked by fluorimetry (like the Qubit): Samples normalised between 4 ng/μl to 40 ng/μl. The higher the concentration, the lower the number of PCR cycles performed.
- Volume: 60 μl
- Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
- Shipment Method: Ice pack or dry ice, overnight
Amplicon Sequencing
- Sample Type: Genomic DNA (RNA-free)
- Sample Purity
- OD260/280: 1.8 - 2.0
- OD260/230: 2.0 – 2.2
- Quality checked on: gel or Genomic Tapestation.
- Recommended Quantity checked by fluorimetry (like the Qubit): 10 ng/µl
- Volume: 25 μl
- Resuspension Buffer: EB, or low TE (<0.1 mM EDTA)
- Shipment Method: Ice pack or dry ice, overnight
Nanopore Whole Genome Sequencing
- Sample Type: Genomic DNA (RNA-free)
- Sample Purity
- OD260/280: 1.8 - 2.0
- OD260/230: 2.0 – 2.2
- Quality checked on: gel or Genomic Tapestation.
- Quantity
- Volume: 48 μl
- Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
- Important information: Avoid use of sample extraction methodologies utilizing phenol,
- Shipment Method: Ice pack or dry ice, overnight
Nanopore Amplicon Sequencing
- Sample Type: Amplicon DNA (RNA-free)
- Sample Purity
- PCR product to be cleaned using e.g. Ampure XP beads (or equivalent), cleanup columns, etc
- Recommended Quantity checked by fluorimetry (like the Qubit): >200fmol
- Minimum quantity: 100fmol (see https://nebiocalculator.neb.com/#!/dsdnaamt )
- Quality checked on: gel or Genomic Tapestation.
- Volume: 12.5 μl
- Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
- Shipment Method: Ice pack or dry ice, overnight
