RNA sample submission guidelines
Poly A isolation (for Eukaryotes only)
- Sample Type: Total RNA (DNA-free)
- Recommended RIN: ≥8.0
- Recommended Quantity: Samples normalised between 1 ng/μl to 40 ng/μl. The higher the concentration, the lower the number of PCR cycles performed.
- Volume: 55 μl
- Resuspension Buffer: Nuclease-free water
- Shipment Method: Dry ice, overnight
rRNA removal (for Prokaryotes mostly)
- Sample Type: Total RNA (DNA-free)
- Recommended RIN: ≥8.0
- Recommended Quantity: Samples normalised between 1 ng/μl to 91 ng/μl. The higher the concentration, the lower the number of PCR cycles performed.
- Volume: 30 μl
- Resuspension Buffer: Nuclease-free water
- Shipment Method: Dry ice, overnight
Small RNA-Seq
- Sample Type: Total RNA (containing small RNA and DNA-free)
- Recommended Quantity: Samples normalised between 1 ng/μl to 400 ng/μl, ideally 200 ng/μl. The higher the concentration, the lower the number of PCR cycles performed.
- Volume: 20 μl
- Resuspension Buffer: Nuclease-free water
- Shipment Method: Dry ice, overnight
Nanopore Long Read RNA-Seq
- Sample Type: Total RNA or polyA
- Sample Purity (OD260/OD280): 1.8-2.2
- Recommended RIN: ≥7.0
- Total RNA
- Recommended Quantity: ≥500 ng, >50 ng/µl
- Minimum Quantity: 250 ng, 20 ng/ul
- PolyA
- Recommended Quantity: ≥10 ng, >1 ng/µl
- Minimum Quantity: 5 ng, 0.5 ng/ul
- Resuspension Buffer: Nuclease-free water
- Shipment Method: Dry ice, overnight
